Method of constructing sequencing library
Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplifi…
Original patent title: “Method of constructing sequencing library”
What this patent covers
The actual claim
Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplification to obtain a whole genome amplification product; 3) fragmenting the whole genome amplification product using a transposase embedded with two adaptors to obtain a fragmented product with two adaptors respectively at two ends; and 4) amplifying the fragmented product with two adaptors respectively at two ends using a tag sequence and a pair of primers to obtain said sequencing library.
What this patent does NOT cover
The boundaries
These exclusions are unique to PatentBrief — derived from the actual claim language, not patent-office boilerplate.
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Patent Abstract
Patent abstract
Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplification to obtain a whole genome amplification product; 3) fragmenting the whole genome amplification product using a transposase embedded with two adaptors to obtain a fragmented product with two adaptors respectively at two ends; and 4) amplifying the fragmented product with two adaptors respectively at two ends using a tag sequence and a pair of primers to obtain said sequencing library.
Patent Journey
From filing to today
Patent Filed
2017
Patent Granted
2019 · 2yr after filing
Active Today
2026
Expires
2037
PatentBrief Score
Impact Score
Early stage
Citation count
6/40
Early citations
Claim breadth
13/20
Broad claims
Recency
10/20
Granted 5–10 years ago
Assignee scale
0/20
Independent or smaller assignee
PatentBrief Impact Score — based on citation count, claim breadth, recency, and assignee scale. Not a legal assessment.
The original legal language
Original claims
20 claims as filed with the patent office.
Citations
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