{
  "patent_number": "US 9839649",
  "country": "US",
  "title": "How to Design Effective siRNA Molecules to Silence Specific Genes",
  "original_title": "Methods and compositions for selecting siRNA of improved functionality",
  "summary": "A patent detailing the precise chemical structure and sequence requirements for siRNA molecules designed to effectively turn off the AAT gene in cells.",
  "what_it_does": "This patent describes the structural requirements for small interfering RNA (siRNA) molecules used to trigger gene silencing. It specifies that the molecule must consist of a sense and antisense strand, each between 19 and 30 nucleotides long, forming a duplex region. Crucially, the antisense strand must be a complement to a specific target sequence identified as SEQ ID NO:465, which corresponds to the AAT gene. The patent allows for variations in the presence or absence of short nucleotide overhangs at the ends of the duplex.",
  "what_it_does_not_cover": [
    "Does not cover siRNA molecules that target genes other than the AAT gene specified by SEQ ID NO:465.",
    "Does not cover RNA molecules longer than 30 nucleotides.",
    "Does not cover single-stranded RNA molecules.",
    "Does not cover methods of delivering these molecules into a living organism."
  ],
  "filed": "2015-11-09",
  "granted": "2017-12-12",
  "expires": "2035-11-09",
  "status": "active",
  "holder": "Thermo Fisher Scientific Inc",
  "holder_url": "https://patentbrief.org/company/thermo-fisher-scientific-inc",
  "inventors": [
    {
      "name": "Devin Leake",
      "url": "https://patentbrief.org/inventor/devin-leake"
    },
    {
      "name": "Anastasia Khvorova",
      "url": "https://patentbrief.org/inventor/anastasia-khvorova"
    },
    {
      "name": "Stephen Scaringe",
      "url": "https://patentbrief.org/inventor/stephen-scaringe"
    },
    {
      "name": "Steven Read",
      "url": "https://patentbrief.org/inventor/steven-read"
    },
    {
      "name": "William Marshall",
      "url": "https://patentbrief.org/inventor/william-marshall"
    },
    {
      "name": "Angela Reynolds",
      "url": "https://patentbrief.org/inventor/angela-reynolds"
    }
  ],
  "times_cited": 3,
  "tags": [
    "biotech",
    "pharmaceutical"
  ],
  "abstract": "Efficient sequence specific gene silencing is possible through the use of siRNA technology. Be selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods compositions, and kits generated through rational design of siRNAs are disclosed, including those directed to the nucleotide sequences for AAT.",
  "url": "https://patentbrief.org/patent/us/9839649/methods-and-compositions-for-selecting-sirna-of-improved-functionality",
  "markdown_url": "https://patentbrief.org/patent/us/9839649/methods-and-compositions-for-selecting-sirna-of-improved-functionality/md",
  "google_patents_url": "https://patents.google.com/patent/US9839649",
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