{
  "patent_number": "US 5397703",
  "country": "US",
  "title": "Using Insect Cells to Create Antibodies for Human Diseases",
  "original_title": "Method for generation of antibodies to cell surface molecules",
  "summary": "A method for creating disease-fighting antibodies by using insect cells to display human proteins, which are then injected into animals to trigger an immune response.",
  "what_it_does": "This patent describes a way to make antibodies against specific proteins found on the surface of human cells. Instead of using complex human cells to produce these proteins for immunization, the inventors use insect cells. They insert DNA into insect cells, which then force the insects to display the human protein on their outer surface. These insect cells are injected into a host animal, like a mouse, which produces antibodies against the human protein. Finally, the researchers screen these antibodies using human cells to ensure they work correctly in a human biological context.",
  "what_it_does_not_cover": [
    "Does not cover the use of mammalian or bacterial cells to express the target antigen for immunization.",
    "Does not cover the production of antibodies using synthetic peptides or purified protein fragments that are not displayed on a cell surface.",
    "Does not cover the use of non-baculoviral vectors for transfecting the insect cells.",
    "Does not cover therapeutic methods or the clinical administration of the resulting antibodies to human patients."
  ],
  "filed": "1992-07-09",
  "granted": "1995-03-14",
  "expires": null,
  "status": "active",
  "holder": "Cetus Oncology Corp",
  "holder_url": "https://patentbrief.org/company/cetus-oncology-corp",
  "inventors": [
    {
      "name": "Leah B. Conroy",
      "url": "https://patentbrief.org/inventor/leah-b-conroy"
    },
    {
      "name": "Mark De Boer",
      "url": "https://patentbrief.org/inventor/mark-de-boer"
    }
  ],
  "times_cited": 25,
  "tags": [
    "biotech",
    "pharmaceutical"
  ],
  "abstract": "The present invention relates to a method of generating antibodies directed against cell surface antigens. The method uses as immunogen recombinant insect cells into which have been transfected coding regions for a molecule containing a cell surface antigen. Host animals are immunized with these transfected insect cells to generate antibodies directed against the cell surface antigen. Antibody-producing cells from the host animal are used to generate monoclonal antibody-producing hybridoma cells. Sera and hybridoma supernatants can be tested for the presence of antibodies against the surface antigen, using the transfected cells in a screening assay.",
  "url": "https://patentbrief.org/patent/us/5397703/hepatitis-c-virus-genome-discovery",
  "markdown_url": "https://patentbrief.org/patent/us/5397703/hepatitis-c-virus-genome-discovery/md",
  "google_patents_url": "https://patents.google.com/patent/US5397703",
  "relatedPatents": []
}