{
  "patent_number": "US 20240384303",
  "country": "US",
  "title": "Improving CRISPR Gene Editing with Intentional DNA Mismatches",
  "original_title": "Genome editing method based on crispr/cas9 system and use thereof",
  "summary": "This patent describes a method to make CRISPR/Cas9 gene editing more effective by intentionally creating small mismatches between the guide RNA and the target DNA sequence.",
  "what_it_does": "This patent details a genome editing method using the CRISPR/Cas9 system that includes a 'donor nucleic acid molecule' and 'guide RNA'. The core idea is to intentionally create 'one or more mismatched nucleotides' between the target DNA and the guide RNA sequence (Claim 1). This mismatch is designed to increase the efficiency of genome editing (Claim 8). The guide RNA can be a single-chain guide RNA (sgRNA) or a dual RNA (crRNA and tracrRNA) (Claim 2). The donor nucleic acid molecule then causes specific genetic changes, such as substituting, inserting, or deleting nucleotides, or even knocking out or knocking in entire sequences (Claim 5, 6). For example, to correct a specific genetic mutation, a guide RNA with a planned mismatch would direct the Cas9 enzyme to the target DNA, and a donor nucleic acid would provide the correct sequence to be inserted.",
  "what_it_does_not_cover": [
    "Does not cover CRISPR/Cas9 genome editing methods that do not involve generating one or more mismatched nucleotides between the target DNA and the guide RNA.",
    "Does not cover gene editing systems that do not rely on the CRISPR/Cas9 mechanism, such as zinc finger nucleases or TALENs.",
    "Does not cover methods where the guide RNA perfectly matches the target DNA sequence without any intentional mismatches.",
    "Does not cover simply delivering CRISPR/Cas9 components to a cell without the specific mismatch strategy described.",
    "Does not cover modifications to the Cas9 enzyme itself to improve specificity or reduce off-target effects, unless those modifications are used in conjunction with the mismatch strategy."
  ],
  "filed": "2021-04-02",
  "granted": null,
  "expires": "2041-04-02",
  "status": "active",
  "holder": "Chung-Ang University",
  "holder_url": "https://patentbrief.org/company/chung-ang-university",
  "inventors": [
    {
      "name": "Hyun Ju Kim",
      "url": "https://patentbrief.org/inventor/hyun-ju-kim"
    },
    {
      "name": "Ho Joung LEE",
      "url": "https://patentbrief.org/inventor/ho-joung-lee"
    },
    {
      "name": "Sang Jun Lee",
      "url": "https://patentbrief.org/inventor/sang-jun-lee"
    }
  ],
  "times_cited": 0,
  "tags": [
    "biotech",
    "pharmaceutical",
    "gene_editing",
    "biotechnology"
  ],
  "abstract": "The present invention pertains to a genome editing method based on a CRISPR/CAS9 system, and a use thereof. A CRISPR system using oligonucleotide-induced mutagenesis and mismatch guide RNA (sgRNA) according to the present invention achieves a significant genome editing effect on target DNA. Thus, it is expected that the CRISPR system of the present invention will be able to be used in a wide range of fields, such as compositions for gene editing using genetic scissors, genome level screening, therapeutic agents for treating various diseases including cancer, the development of compositions for disease diagnosis or imaging, and the development of transgenic plants and animals.",
  "url": "https://patentbrief.org/patent/us/20240384303/genome-editing-method-based-on-crisprcas9-system-and-use-thereof",
  "markdown_url": "https://patentbrief.org/patent/us/20240384303/genome-editing-method-based-on-crisprcas9-system-and-use-thereof/md",
  "google_patents_url": "https://patents.google.com/patent/US20240384303",
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}