# How to Design High-Quality Gene Editing Tools for a Whole Genome > This patent describes a computer system and method for creating a comprehensive library of gene-editing guides (sgRNAs) by carefully designing and filtering them to be accurate and avoid unintended genetic changes. - **Patent:** US 20230187025 - **Original title:** Whole genome sgrna library constructing system and application thereof - **Owner:** Genewiz Suzhou - **Status:** Active - **Times cited:** 1 - **Field:** biotech, gene_editing, software ## What it does The patent details a system for building a "genome-wide sgRNA library," which are small RNA molecules used to guide gene-editing tools like CRISPR. The system has three main parts. First, an "input module" downloads genetic information, like DNA sequences and gene descriptions, from a database and picks specific gene regions (such as commonly deleted segments or CDS for protein-coding genes, per claim 2) to target. Second, an "sgRNA design module" creates many potential sgRNAs, checking them against the entire genome (claim 1). It looks for how well they match and assigns a risk score based on how likely they are to hit unintended spots (off-target sites), using rules like allowing 3 to 6 mismatches (claim 4). Finally, an "sgRNA filtering module" cleans up this list, removing problematic sgRNAs (like those with four or more consecutive identical bases, per claim 1) and ensuring the remaining ones are spread out evenly across the target genes (claim 1). For example, if a scientist wanted to study every gene in a human cell, this system could generate a precise set of sgRNAs, each designed to target a specific gene without accidentally editing other genes. ## What it does NOT cover - Does not cover systems that design sgRNAs without evaluating off-target rates or grading them by risk (claim 1, 4). - Does not cover systems that do not filter sgRNAs based on consecutive base repeats, overlap, or even distribution on a CDS (claim 1). - Does not cover methods of constructing sgRNA libraries that do not use a 20 nucleotide guide sequence combined with a specific PAM sequence (NGG or GGN) (claim 1). - Does not cover systems that only target non-coding regions and do not extract CDS sequences for protein-encoding genes (claim 1, 2). - Does not cover the actual chemical synthesis or biological delivery of the sgRNA molecules themselves into a cell. ## The clever bit The patent's novelty lies in its systematic, multi-stage approach to designing and filtering sgRNAs, combining specific criteria for off-target evaluation (like penalty points for mismatch positions) and rigorous filtering (like ensuring even distribution and no overlaps) to produce a high-quality, genome-wide library. This integrated system aims to drastically reduce the number of ineffective or harmful sgRNAs. ## Real-world examples 1. CRISPR screening platforms 2. Academic gene function studies 3. Drug discovery pipelines using genetic screens 4. Biotechnology companies developing gene therapies ## Why it matters This technology is crucial for advancing gene editing research, particularly with CRISPR-Cas9 systems. By creating highly accurate and specific sgRNA libraries, scientists can perform large-scale genetic screens to understand gene function, identify drug targets, and develop gene therapies more efficiently. High-quality sgRNA design minimizes unintended edits, which is vital for both research validity and therapeutic safety. ## Frequently asked questions ### What does How to Design High-Quality Gene Editing Tools for a Whole Genome cover? This patent describes a computer system and method for creating a comprehensive library of gene-editing guides (sgRNAs) by carefully designing and filtering them to be accurate and avoid unintended genetic changes. ### Who owns patent US 20230187025? This patent is owned by Genewiz Suzhou. ### When does this patent expire? This patent is expected to expire on December 14, 2038, when the invention enters the public domain. ### What is patent US 20230187025 cited by? This patent has been cited by 1 later patents that build on its ideas. ### What problem does this patent solve? This technology is crucial for advancing gene editing research, particularly with CRISPR-Cas9 systems. By creating highly accurate and specific sgRNA libraries, scientists can perform large-scale genetic screens to understand gene function, identify drug targets, and develop gene therapies more efficiently. High-quality sgRNA design minimizes unintended edits, which is vital for both research validity and therapeutic safety. ### What does this patent NOT cover? Does not cover systems that design sgRNAs without evaluating off-target rates or grading them by risk (claim 1, 4). **Full plain-English explainer:** https://patentbrief.org/patent/us/20230187025/whole-genome-sgrna-library-constructing-system-and-application-thereof **Original patent:** https://patents.google.com/patent/US20230187025 --- _Source: PatentBrief — https://patentbrief.org. Patent facts are from public records; the plain-English explanation is PatentBrief's._ ## Related patents Semantically similar inventions in the PatentBrief corpus: - [How to Edit Genes in Human Cells Using an Engineered CRISPR System](https://patentbrief.org/patent/us/8697359/crispr-gene-editing) — This patent describes an engineered CRISPR-Cas9 system for precisely cutting DNA in eukaryotic cells to change how genes work, opening the door for gene editing in complex organisms. - [How to Precisely Edit Genes Using Retron-Guide RNA Cassettes](https://patentbrief.org/patent/us/20230383290/high-throughput-precision-genome-editing) — This patent describes a method for highly efficient and precise genome editing using a retron-guide RNA cassette to deliver large pieces of donor DNA into a cell's genetic material. - [How CRISPR-Cas9 Uses RNA to Edit DNA](https://patentbrief.org/patent/us/10113167/methods-and-compositions-for-rna-directed-target-dna-modification-and-for-rna-directed-modulation-of-transcription) — This patent describes the fundamental mechanism of using a two-part RNA system to guide the Cas9 protein to specific locations in DNA for precise editing. - [How to Make Animals That Can Turn On CRISPR Gene Editing](https://patentbrief.org/patent/us/12252707/delivery-engineering-and-optimization-of-systems-methods-and-compositions-for-se) — This patent describes creating genetically modified non-human animals, like mice, where a key CRISPR gene-editing protein (Cas9) can be turned on only in specific cells or at specific times using a special genetic switch. - [Highly Efficient Bacterial Gene Editing Using Guide RNA and Reverse Transcriptase](https://patentbrief.org/patent/us/20180127759/dynamic-genome-engineering) — This patent describes a system for precisely editing the DNA of bacterial cells with very high success rates, using a combination of guide RNA, reverse transcriptase, and specific DNA sequences.