{
  "patent_number": "US 20230187025",
  "country": "US",
  "title": "How to Design High-Quality Gene Editing Tools for a Whole Genome",
  "original_title": "Whole genome sgrna library constructing system and application thereof",
  "summary": "This patent describes a computer system and method for creating a comprehensive library of gene-editing guides (sgRNAs) by carefully designing and filtering them to be accurate and avoid unintended genetic changes.",
  "what_it_does": "The patent details a system for building a \"genome-wide sgRNA library,\" which are small RNA molecules used to guide gene-editing tools like CRISPR. The system has three main parts. First, an \"input module\" downloads genetic information, like DNA sequences and gene descriptions, from a database and picks specific gene regions (such as commonly deleted segments or CDS for protein-coding genes, per claim 2) to target. Second, an \"sgRNA design module\" creates many potential sgRNAs, checking them against the entire genome (claim 1). It looks for how well they match and assigns a risk score based on how likely they are to hit unintended spots (off-target sites), using rules like allowing 3 to 6 mismatches (claim 4). Finally, an \"sgRNA filtering module\" cleans up this list, removing problematic sgRNAs (like those with four or more consecutive identical bases, per claim 1) and ensuring the remaining ones are spread out evenly across the target genes (claim 1). For example, if a scientist wanted to study every gene in a human cell, this system could generate a precise set of sgRNAs, each designed to target a specific gene without accidentally editing other genes.",
  "what_it_does_not_cover": [
    "Does not cover systems that design sgRNAs without evaluating off-target rates or grading them by risk (claim 1, 4).",
    "Does not cover systems that do not filter sgRNAs based on consecutive base repeats, overlap, or even distribution on a CDS (claim 1).",
    "Does not cover methods of constructing sgRNA libraries that do not use a 20 nucleotide guide sequence combined with a specific PAM sequence (NGG or GGN) (claim 1).",
    "Does not cover systems that only target non-coding regions and do not extract CDS sequences for protein-encoding genes (claim 1, 2).",
    "Does not cover the actual chemical synthesis or biological delivery of the sgRNA molecules themselves into a cell."
  ],
  "filed": "2018-12-14",
  "granted": null,
  "expires": "2038-12-14",
  "status": "active",
  "holder": "Genewiz Suzhou",
  "holder_url": "https://patentbrief.org/company/genewiz-suzhou",
  "inventors": [
    {
      "name": "Wenyan Zhao",
      "url": "https://patentbrief.org/inventor/wenyan-zhao"
    },
    {
      "name": "Liang Jin",
      "url": "https://patentbrief.org/inventor/liang-jin"
    },
    {
      "name": "Fengdan Xu",
      "url": "https://patentbrief.org/inventor/fengdan-xu"
    },
    {
      "name": "Yi Ge",
      "url": "https://patentbrief.org/inventor/yi-ge"
    },
    {
      "name": "Guangyou Duan",
      "url": "https://patentbrief.org/inventor/guangyou-duan"
    },
    {
      "name": "Pengyang Xu",
      "url": "https://patentbrief.org/inventor/pengyang-xu"
    }
  ],
  "times_cited": 1,
  "tags": [
    "biotech",
    "gene_editing",
    "software"
  ],
  "abstract": "Provided are a system for constructing a genome-wide sgRNA library and a use thereof. The system includes an input module, an sgRNA design module and an sgRNA filtering module. By constructing three modules in the system, optimizing details and processes in the modules, and adopting multiple design criteria and screening principles, the genome-wide sgRNA library is finally constructed. The system and method are concise and efficient, and the obtained library has a high quality and good activity, and is convenient for applications in gene editing researches.",
  "url": "https://patentbrief.org/patent/us/20230187025/whole-genome-sgrna-library-constructing-system-and-application-thereof",
  "markdown_url": "https://patentbrief.org/patent/us/20230187025/whole-genome-sgrna-library-constructing-system-and-application-thereof/md",
  "google_patents_url": "https://patents.google.com/patent/US20230187025",
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}