{
  "patent_number": "US 20210055304",
  "country": "US",
  "title": "How to Measure Unbound Drug Target in a Sample",
  "original_title": "Method for the detection of a binding partner of a multispecific binder",
  "summary": "This patent describes a laboratory method for accurately measuring the amount of a specific target molecule (antigen) that is not yet bound by a complex drug (multispecific antibody) in a sample, which is important for drug development.",
  "what_it_does": "This method helps scientists determine the amount of 'free antigen' in a sample that also contains a 'multispecific antibody' drug. First, the sample is mixed with a 'second antigen' that specifically attaches to a different part of the multispecific antibody, forming a 'second antigen-multispecific antibody complex' (Claim 11). This complex is then removed from the sample (Claim 11), leaving behind a 'multispecific antibody-depleted sample'. Finally, the amount of the original free antigen in this depleted sample is measured (Claim 14). For example, if a drug binds to both a cancer cell marker and a blood protein, this method allows researchers to precisely measure how much of the cancer cell marker is still available, not yet bound by the drug.",
  "what_it_does_not_cover": [
    "Does not cover methods for detecting free antigen of a monospecific antibody (an antibody that only binds to one type of target).",
    "Does not cover methods that measure the total amount of antigen, only the *free* or unbound antigen.",
    "Does not cover methods that do not involve removing the multispecific antibody from the sample before measuring the free antigen.",
    "Does not cover methods where the multispecific antibody is depleted using an anti-idiotypic antibody instead of a 'second antigen'.",
    "Does not cover methods where the second antigen binds to the *same* binding specificity as the first antigen."
  ],
  "filed": "2020-07-31",
  "granted": null,
  "expires": "2040-07-31",
  "status": "active",
  "holder": "Hoffmann La Roche",
  "holder_url": "https://patentbrief.org/company/hoffmann-la-roche",
  "inventors": [
    {
      "name": "Kay-Gunnar Stubenrauch",
      "url": "https://patentbrief.org/inventor/kay-gunnar-stubenrauch"
    },
    {
      "name": "Uwe Wessels",
      "url": "https://patentbrief.org/inventor/uwe-wessels"
    }
  ],
  "times_cited": 0,
  "tags": [
    "biotech",
    "pharmaceutical",
    "diagnostics"
  ],
  "abstract": "Herein is reported a method for the detection of free antigen of a multispecific antibody in a sample, whereby the antigen to be detected can be specifically bound by a first binding site of the multispecific antibody, comprising the step of incubating a sample comprising free antigen and multispecific antibody with an anti-idiotypic antibody, which specifically binds to a second binding specificity of the bispecific antibody, which is different from the first binding specificity, whereby the anti-idiotypic antibody is bound to a solid phase.",
  "url": "https://patentbrief.org/patent/us/20210055304/method-for-the-detection-of-a-binding-partner-of-a-multispecific-binder",
  "markdown_url": "https://patentbrief.org/patent/us/20210055304/method-for-the-detection-of-a-binding-partner-of-a-multispecific-binder/md",
  "google_patents_url": "https://patents.google.com/patent/US20210055304",
  "relatedPatents": []
}