{
  "patent_number": "US 20180127759",
  "country": "US",
  "title": "Highly Efficient Bacterial Gene Editing Using Guide RNA and Reverse Transcriptase",
  "original_title": "Dynamic genome engineering",
  "summary": "This patent describes a system for precisely editing the DNA of bacterial cells with very high success rates, using a combination of guide RNA, reverse transcriptase, and specific DNA sequences.",
  "what_it_does": "This patent describes an engineered nucleic acid construct designed for highly efficient gene editing in bacterial cells. The construct includes three main parts: a nucleotide sequence encoding a guide RNA that targets an exonuclease (like RecJ, XonA, or ExoX, as per claim 5), a sequence for a modified single-stranded msrRNA and msdDNA containing a specific targeting sequence flanked by inverted repeats (claim 1b), and a sequence for a reverse transcriptase protein (claim 1c). When delivered to a bacterial cell, this system works to modify specific target nucleotide sequences, such as an undesired allele of a gene (claim 20), by using the guide RNA to disable exonucleases and the reverse transcriptase to help incorporate the new DNA, leading to nearly 100% recombination efficiency.",
  "what_it_does_not_cover": [
    "Does not cover gene editing systems that do not include a guide RNA specifically targeting an exonuclease.",
    "Does not cover gene editing in eukaryotic cells, as the claims specify bacterial cells.",
    "Does not cover methods that lack a reverse transcriptase protein as a component of the engineered construct.",
    "Does not cover systems where the single-stranded msrRNA and msdDNA targeting sequence is not flanked by inverted repeat sequences.",
    "Does not cover gene editing approaches that rely solely on CRISPR-Cas9 without the additional components like exonuclease targeting and reverse transcriptase."
  ],
  "filed": "2017-10-27",
  "granted": null,
  "expires": "2037-10-27",
  "status": "active",
  "holder": "Massachusetts Institute of Technology",
  "holder_url": "https://patentbrief.org/company/massachusetts-institute-of-technology",
  "inventors": [
    {
      "name": "Timothy Kuan-Ta Lu",
      "url": "https://patentbrief.org/inventor/timothy-kuan-ta-lu"
    },
    {
      "name": "Fahim Farzadfard",
      "url": "https://patentbrief.org/inventor/fahim-farzadfard"
    }
  ],
  "times_cited": 30,
  "tags": [
    "biotech",
    "pharmaceutical",
    "synthetic_biology",
    "materials",
    "food_and_agriculture"
  ],
  "abstract": "Provided herein, in some embodiments, are genomic editing constructs that can achieve nearly 100% recombination efficiency within a select population of bacterial cells.",
  "url": "https://patentbrief.org/patent/us/20180127759/dynamic-genome-engineering",
  "markdown_url": "https://patentbrief.org/patent/us/20180127759/dynamic-genome-engineering/md",
  "google_patents_url": "https://patents.google.com/patent/US20180127759",
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}