{
  "patent_number": "US 12735746",
  "country": "US",
  "title": "How to Amplify Many DNA Targets at Once, Cleanly",
  "original_title": "Methods for simultaneous amplification of target loci",
  "summary": "This patent describes methods for simultaneously making many copies of different DNA sections in a single test tube, along with ways to choose the right starting pieces (primers) to avoid unwanted byproducts.",
  "what_it_does": "The invention provides techniques for amplifying multiple specific nucleic acid regions of interest at the same time within one reaction volume. This means a scientist can test for many different DNA sequences in a single experiment, rather than running separate tests for each. A key part of the method involves carefully selecting a library of primers, which are short DNA pieces that start the copying process. This selection aims to minimize the creation of unwanted amplified primer dimers or other non-target amplicons, ensuring that only the desired DNA regions are copied efficiently. For example, a single test could detect several different viral strains in a patient sample simultaneously.",
  "what_it_does_not_cover": [
    "Does not cover methods that amplify only a single nucleic acid region in a reaction.",
    "Does not cover methods that amplify multiple regions but require separate reaction volumes for each target.",
    "Does not cover amplification methods that do not specifically focus on minimizing primer dimers or other non-target amplicons.",
    "Does not cover techniques for detecting nucleic acids without an amplification step.",
    "Does not cover methods for sequencing nucleic acids without an initial amplification."
  ],
  "filed": "2022-07-19",
  "granted": "2026-09-15",
  "expires": null,
  "status": "active",
  "holder": null,
  "holder_url": null,
  "inventors": [],
  "times_cited": 0,
  "tags": [
    "biotech",
    "pharmaceutical",
    "telecommunications",
    "diagnostics",
    "gene_editing"
  ],
  "abstract": "The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.",
  "url": "https://patentbrief.org/patent/us/12735746/methods-for-simultaneous-amplification-of-target-loci",
  "markdown_url": "https://patentbrief.org/patent/us/12735746/methods-for-simultaneous-amplification-of-target-loci/md",
  "google_patents_url": "https://patents.google.com/patent/US12735746",
  "relatedPatents": []
}