{
  "patent_number": "US 12735743",
  "country": "US",
  "title": "Mapping Tiny Features on a Chip with DNA Barcodes",
  "original_title": "Resolving spatial arrays",
  "summary": "This patent describes a method for precisely locating individual biological features, like cells or molecules, on a prepared surface by using unique DNA tags and imaging techniques.",
  "what_it_does": "The patent describes a method for finding the exact spot of a \"feature\" on a \"spatial array\" (a surface with many tiny spots). First, the array is prepared with features, each having a \"capture probe\" that includes a unique \"spatial barcode\" and a constant DNA sequence. Next, a \"sequencing probe\" with a fluorescent \"label\" attaches to the spatial barcode. An image is then taken of this label. By looking at the image, a part of the spatial barcode's sequence is figured out. Finally, the feature is linked to its exact location on the array based on where its label appeared in the image. This allows researchers to know not just what a feature is, but precisely where it is.",
  "what_it_does_not_cover": [
    "Does not cover methods for determining feature location without using specific DNA spatial barcodes.",
    "Does not cover identifying the full genetic sequence of a feature, only a portion of its spatial barcode.",
    "Does not cover systems that rely solely on physical coordinates without molecular tags for localization.",
    "Does not cover methods where the sequencing probe does not hybridize to a spatial barcode."
  ],
  "filed": "2019-08-27",
  "granted": "2026-09-15",
  "expires": null,
  "status": "active",
  "holder": null,
  "holder_url": null,
  "inventors": [],
  "times_cited": 0,
  "tags": [
    "biotech",
    "software",
    "materials",
    "life_sciences"
  ],
  "abstract": "Methods for determining a location of a feature in a spatial array of features include: providing a spatial array including a plurality of features on a substrate, where a feature of the plurality of features has a capture probe, and where the capture probe has a spatial barcode and a constant sequence; hybridizing a first sequencing probe to the spatial barcode, where the first sequencing probe has a first label and a first nucleotide sequence; obtaining a first image of the first label with the first sequencing probe hybridized to the spatial barcode; determining, based on the first image, a first portion of a sequence of the spatial barcode; and associating the feature with a location in the spatial array based on a location of the first label in the first image.",
  "url": "https://patentbrief.org/patent/us/12735743/resolving-spatial-arrays",
  "markdown_url": "https://patentbrief.org/patent/us/12735743/resolving-spatial-arrays/md",
  "google_patents_url": "https://patents.google.com/patent/US12735743",
  "relatedPatents": []
}