{
  "patent_number": "US 11739322",
  "country": "US",
  "title": "CRISPR Gene Editing That Turns Itself Off",
  "original_title": "Method for genome editing using a self-inactivating CRISPR nuclease",
  "summary": "This patent describes a CRISPR gene editing system that turns itself off after making a specific genetic change, using a second guide RNA to inactivate the editing enzyme.",
  "what_it_does": "This patent describes a genome editing system that precisely modifies a target gene and then deactivates itself. It uses three main parts: a first 'guide RNA' (gRNA) that directs a CRISPR nuclease to the specific gene needing modification, the CRISPR nuclease itself which performs the editing, and a second gRNA. This second gRNA is designed to target a sequence within the CRISPR nuclease's own genetic code. Once introduced into a cell, the nuclease first makes the desired changes to the target gene. Then, the second gRNA guides the nuclease to its own coding sequence, causing an 'inactivating mutation' that effectively breaks the nuclease, preventing it from making further, potentially unwanted, edits. For example, a scientist could use this system to correct a single faulty base in a gene, and then the editing tool would self-destruct.",
  "what_it_does_not_cover": [
    "Does not cover CRISPR systems that remain active indefinitely after making their intended genetic changes.",
    "Does not cover gene editing systems that lack the specific 'second gRNA' designed to target and inactivate the CRISPR nuclease itself.",
    "Does not cover systems where the inactivating mutation is not within the coding sequence of the CRISPR nuclease, as specified in claim 1.",
    "Does not cover CRISPR nucleases that are not 'single-base editing' nucleases, as specified in claim 1.",
    "Does not cover systems that do not use three separate expression constructs for the gRNAs and the CRISPR nuclease."
  ],
  "filed": "2019-01-31",
  "granted": "2023-08-29",
  "expires": "2039-01-31",
  "status": "active",
  "holder": "Institute of Genetics and Developmental Biology of CAS",
  "holder_url": "https://patentbrief.org/company/institute-of-genetics-and-developmental-biology-of-cas",
  "inventors": [
    {
      "name": "Huawei ZHANG",
      "url": "https://patentbrief.org/inventor/huawei-zhang"
    },
    {
      "name": "Caixia GAO",
      "url": "https://patentbrief.org/inventor/caixia-gao"
    },
    {
      "name": "Shuai JIN",
      "url": "https://patentbrief.org/inventor/shuai-jin"
    }
  ],
  "times_cited": 0,
  "tags": [
    "biotech",
    "gene_editing",
    "pharmaceutical",
    "agriculture"
  ],
  "abstract": "Provided are an improved genome editing system and a method that has high specificity, which are capable of obtaining stable mutation types. The system includes an expression construct including a coding sequence of a gRNA targeting at least one genomic target sequence; an expression construct including a coding sequence of a CRISPR nuclease; and an expression construct including a coding sequence for a gRNA targeting a target sequence within the coding sequence of the CRISPR nuclease. Upon introduction into the cell, the gRNA targeting the at least one genomic target sequence directs the CRISPR nuclease to the at least one genomic target sequence and results in one or more mutations in the genomic target sequence, and the gRNA targeting a target sequence within the coding sequence of the CRISPR nuclease directs the CRISPR nuclease to the target sequence within the coding sequence of the CRISPR nuclease and results in an inactivating mutation of the CRISPR nuclease.",
  "url": "https://patentbrief.org/patent/us/11739322/method-for-genome-editing-using-a-self-inactivating-crispr-nuclease",
  "markdown_url": "https://patentbrief.org/patent/us/11739322/method-for-genome-editing-using-a-self-inactivating-crispr-nuclease/md",
  "google_patents_url": "https://patents.google.com/patent/US11739322",
  "relatedPatents": [
    {
      "patentNumber": "8697359",
      "countryCode": "US",
      "title": "How to Edit Genes in Human Cells Using an Engineered CRISPR System",
      "url": "https://patentbrief.org/patent/us/8697359/crispr-gene-editing"
    },
    {
      "patentNumber": "10113167",
      "countryCode": "US",
      "title": "How CRISPR-Cas9 Uses RNA to Edit DNA",
      "url": "https://patentbrief.org/patent/us/10113167/methods-and-compositions-for-rna-directed-target-dna-modification-and-for-rna-directed-modulation-of-transcription"
    },
    {
      "patentNumber": "20230383290",
      "countryCode": "US",
      "title": "How to Precisely Edit Genes Using Retron-Guide RNA Cassettes",
      "url": "https://patentbrief.org/patent/us/20230383290/high-throughput-precision-genome-editing"
    },
    {
      "patentNumber": "12252707",
      "countryCode": "US",
      "title": "How to Make Animals That Can Turn On CRISPR Gene Editing",
      "url": "https://patentbrief.org/patent/us/12252707/delivery-engineering-and-optimization-of-systems-methods-and-compositions-for-se"
    },
    {
      "patentNumber": "20180305704",
      "countryCode": "US",
      "title": "How to Use Many CRISPR Guides to Study Many Genes at Once",
      "url": "https://patentbrief.org/patent/us/20180305704/crispr-cas-component-systems-methods-and-compositions-for-sequence-manipulation"
    }
  ]
}