{
  "patent_number": "US 10456769",
  "country": "US",
  "title": "How to Sequence DNA from Tiny Samples Using Specialized Plates",
  "original_title": "Method of constructing sequencing library",
  "summary": "A method for preparing DNA for genetic sequencing by splitting tiny samples into a 5184-well plate to ensure accurate data from very few cells.",
  "what_it_does": "This patent describes a specific workflow for preparing DNA for high-throughput sequencing, designed to handle samples with as few as 10 to 500 cells. The process involves distributing single-stranded DNA into a 5184-well plate, where the volume of each well is strictly controlled to 350 nL. By keeping the sample size small and the distribution precise, the method ensures that homologous chromosome fragments from both parents are unlikely to end up in the same well. After amplification and fragmentation using a transposase enzyme, the resulting DNA library is ready for sequencing.",
  "what_it_does_not_cover": [
    "Does not cover sequencing methods that do not use the specific 5184-well plate configuration.",
    "Does not cover general whole genome amplification techniques performed outside of this specific plate-based workflow.",
    "Does not cover sequencing libraries with lengths outside the 550 bp to 1000 bp range specified in the claims.",
    "Does not cover workflows where the probability of homologous chromosome overlap exceeds 1%."
  ],
  "filed": "2017-08-03",
  "granted": "2019-10-29",
  "expires": "2037-08-03",
  "status": "active",
  "holder": "Bgi Shenzhen & Bgi Shenzhen Co Ltd",
  "holder_url": "https://patentbrief.org/company/bgi-shenzhen-bgi-shenzhen-co-ltd",
  "inventors": [
    {
      "name": "Xiaofang Cheng",
      "url": "https://patentbrief.org/inventor/xiaofang-cheng"
    },
    {
      "name": "Cankun Chang",
      "url": "https://patentbrief.org/inventor/cankun-chang"
    },
    {
      "name": "Ou Wang",
      "url": "https://patentbrief.org/inventor/ou-wang"
    },
    {
      "name": "Wenwei Zhang",
      "url": "https://patentbrief.org/inventor/wenwei-zhang"
    },
    {
      "name": "Hui Jiang",
      "url": "https://patentbrief.org/inventor/hui-jiang"
    },
    {
      "name": "Liangying Zou",
      "url": "https://patentbrief.org/inventor/liangying-zou"
    }
  ],
  "times_cited": 1,
  "tags": [
    "biotech",
    "semiconductors"
  ],
  "abstract": "Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplification to obtain a whole genome amplification product; 3) fragmenting the whole genome amplification product using a transposase embedded with two adaptors to obtain a fragmented product with two adaptors respectively at two ends; and 4) amplifying the fragmented product with two adaptors respectively at two ends using a tag sequence and a pair of primers to obtain said sequencing library.",
  "url": "https://patentbrief.org/patent/us/10456769/method-of-constructing-sequencing-library",
  "markdown_url": "https://patentbrief.org/patent/us/10456769/method-of-constructing-sequencing-library/md",
  "google_patents_url": "https://patents.google.com/patent/US10456769",
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}